TIP120A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49918
![Western Blot: TIP120A Antibody [NBP1-49918] Western Blot: TIP120A Antibody [NBP1-49918]](https://resources.bio-techne.com/images/products/TIP120A-Antibody-Western-Blot-NBP1-49918-img0001.jpg)
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Bovine (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1180 and 1230 of human Cullin-Associated and Neddylation-Dissociated 1 using the numbering given in entry NP_060918.2 (GeneID 55832).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TIP120A Antibody - BSA Free
Western Blot: TIP120A Antibody [NBP1-49918]
Western Blot: TIP120A Antibody [NBP1-49918] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TIP120A antibody NBP1-49918 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. TIP120A was also immunoprecipitated by rabbit anti-TIP120A antibody NBP1-49917, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Immunohistochemistry-Paraffin: TIP120A Antibody [NBP1-49918]
Immunohistochemistry-Paraffin: TIP120A Antibody [NBP1-49918] - Section of human ovarian carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-CAND1 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DABApplications for TIP120A Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:5000
Immunohistochemistry-Paraffin
1:1:1000 - 1:5000
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TIP120A
Alternate Names
cullin-associated and neddylation-dissociated 1, Cullin-associated and neddylation-dissociated protein 1, cullin-associated NEDD8-dissociated protein 1, DKFZp434M1414, FLJ90441, KIAA0829FLJ10114, p120 CAND1, TBP interacting protein, TBP-interacting protein 120A, TBP-interacting protein of 120 kDa A, TIP120AFLJ38691, TIP120FLJ10929
Gene Symbol
CAND1
Additional TIP120A Products
Product Documents for TIP120A Antibody - BSA Free
Product Specific Notices for TIP120A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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