Thyroglobulin Antibody (CL0164) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44463

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL0164

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Thyroglobulin Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P01266, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Thyroglobulin Antibody (CL0164) - Azide and BSA Free

Thyroglobulin Antibody (CL0164) - Azide and BSA Free Immunohistochemistry-Paraffin: Thyroglobulin Antibody [NBP3-44463]

Immunohistochemistry-Paraffin: Thyroglobulin Antibody [NBP3-44463]

Analysis in human thyroid gland and liver tissues using NBP3-44463 antibody. Corresponding Thyroglobulin RNA-seq data are presented for the same tissues.
Thyroglobulin Antibody (CL0164) - Azide and BSA Free Western Blot: Thyroglobulin Antibody [NBP3-44463]

Western Blot: Thyroglobulin Antibody [NBP3-44463]

Lane 1: Marker [kDa]
Lane 2: Human thyroid tissue lysate
Thyroglobulin Antibody (CL0164) - Azide and BSA Free Immunohistochemistry-Paraffin: Thyroglobulin Antibody [NBP3-44463]

Immunohistochemistry-Paraffin: Thyroglobulin Antibody [NBP3-44463]

Staining of human liver shows no positivity in hepatocytes as expected.
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Applications for Thyroglobulin Antibody (CL0164) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thyroglobulin

Thyroglobulin is synthesized by the follicular epithelial cells of the thyroid. Thyroglobulin is the glycoprotein precursor to the thyroid hormones. Its synthesis and metabolism have seemingly wasteful features. It has a molecular weight of 660,000, with 2 identical subunits of MW 300,000 and 10% sugars; yet its complete hydrolysis yields only 2 to 4 molecules of the iodothyroxines T4 and T3. Baas et al. (1986) showed that the TG gene encodes an 8.7-kb mRNA, covers at least 300 kb of genomic DNA and contains at least 37 exons separated by introns as large as 64 kb. A striking structural difference between the 5-prime and 3-prime parts of the gene suggested that it is composed of 2 evolutionarily different regions. The first 30 kb of DNA encodes 3 kb of the mRNA yielding an exon:intron ratio of 1:10, whereas the remaining 270 kb encodes 5.7 kb of the mRNA with a ratio of 1.47.

Alternate Names

AITD3, cog, TDH3, Tg, TGN

Gene Symbol

TG

Additional Thyroglobulin Products

Product Documents for Thyroglobulin Antibody (CL0164) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Thyroglobulin Antibody (CL0164) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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