Thioredoxin-1 Antibody (116) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89458
Recombinant Monoclonal Antibody
![Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458]](https://resources.bio-techne.com/images/products/Thioredoxin-1-Antibody-116-Western-Blot-NBP2-89458-img0001.jpg "Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 116 expressed in HEK293
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Thioredoxin-1 (Uniprot#: P10599; Met1-Val105).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Thioredoxin-1 Antibody (116) - Azide and BSA Free
Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458]
Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458] - Lane A: Hela Whole Cell Lysate Lane B: THP-1 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:12 kDa Observed band size:14 kDa![Immunoprecipitation: Thioredoxin-1 Antibody (116) [NBP2-89458]](https://resources.bio-techne.com/images/products/Thioredoxin-1-Antibody-116-Immunoprecipitation-NBP2-89458-img0002.jpg "Immunoprecipitation: Thioredoxin-1 Antibody (116) [NBP2-89458]")
Immunoprecipitation: Thioredoxin-1 Antibody (116) [NBP2-89458]
Immunoprecipitation: Thioredoxin-1 Antibody (116) [NBP2-89458] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate2 uL anti-TXN rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-TXN rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 13 kDaApplications for Thioredoxin-1 Antibody (116) - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
4-6 uL/mg of lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Thioredoxin-1
Thioredoxins (Trxs) are a group of small ubiquitous proteins that are key regulators of cellular redox balance. The mammalian Trx family includes the secreted and cellular protein (Trx-1), the mitochondria-specific Trx-2, the Trx-like cytosolic protein p32TrxL, a truncated version of Trx-1 known as Trx80, and the thioredoxin-related protein, TRP-14. Trx-1 is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Trx-1 acts as a growth factor an, antioxidant, a cofactor that provides reducing equivalents, and a transcriptional regulator. Expression of Trx-1 is increased under various stress conditions such as hypoxia and viral and bacterial infections. It also serves as a biomarker for rheumatoid arthritis.
Alternate Names
Thioredoxin1, Trx1, TXN, TXN1
Gene Symbol
TXN
Additional Thioredoxin-1 Products
Product Documents for Thioredoxin-1 Antibody (116) - Azide and BSA Free
Product Specific Notices for Thioredoxin-1 Antibody (116) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...