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THAP7 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81232

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NBP1-81232-25ul
NBP1-81232

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GISFHRLPKKDNPRRGLWLANCQRLDPSGQGLWDPASEYIYFCSKHFEEDCFELVGISGYHRLKEGAVPTIFESFSKLRRTTKTKGHSYPPGPPEVSRLRRCRKRCSEGRGPTTPFSPPPPADVTCFP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%). Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for THAP7 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: THAP7 Antibody [NBP1-81232]

Immunocytochemistry/ Immunofluorescence: THAP7 Antibody [NBP1-81232]

Immunocytochemistry/Immunofluorescence: THAP7 Antibody [NBP1-81232] - Staining of human cell line A-431 shows localization to nuclear membrane & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: THAP7 Antibody [NBP1-81232]

Immunohistochemistry-Paraffin: THAP7 Antibody [NBP1-81232]

Immunohistochemistry-Paraffin: THAP7 Antibody [NBP1-81232] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
THAP7 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: THAP7 Antibody - BSA Free [NBP1-81232]

Chromatin Immunoprecipitation-exo-Seq: THAP7 Antibody - BSA Free [NBP1-81232]

ChIP-Exo-Seq composite graph for Anti-THAP7 (NBP1-81232) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for THAP7 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: THAP7

Chromatin-associated, histone tail-binding protein that represses transcription via recruitment of HDAC3 andnuclear hormone receptor corepressors

Alternate Names

MGC10963, THAP domain containing 7, THAP domain-containing protein 7

Entrez Gene IDs

80764 (Human)

Gene Symbol

THAP7

UniProt

Additional THAP7 Products

Product Documents for THAP7 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for THAP7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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