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TGF-beta 2 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB612

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MAB612-100
MAB612-500
MAB612-SP

Key Product Details

Species Reactivity

Multi-Species

Applications

ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 8607

Product Summary for TGF-beta 2 Antibody

Immunogen

Porcine TGF-beta 1.2

Specificity

Detects TGF-beta 2 from multiple species in ELISAs and Western blots. In ELISAs, no cross-reactivity or interfereence with recombinant human (rh) TGF-alpha, recombinant porcineTGF-beta 1, rhTGF-beta 1, recombinant canine TGF-beta 3, recombinant amphibian TGF-beta 5, rhTGF-beta RII, or rhTGF-beta RIII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for TGF-beta 2 Antibody

TGF-beta 2 antibody in Human Prostate Cancer by Immunohistochemistry (IHC-P).

TGF‑ beta2 in Human Prostate Cancer.

TGF-beta 2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-TGF-beta 2 Monoclonal Antibody (Catalog # MAB612) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for TGF-beta 2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample:  Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

1 µg/mL
Sample: Recombinant Human TGF-beta 2 (Catalog # 302-B2)
under non-reducing conditions only

Human TGF-beta 2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: TGF-beta 2 Biotinylated Antibody (Catalog # BAF302)
  • Standard: Recombinant Human TGF-beta 2 Protein (Catalog # 302-B2)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using MAB612 in the following applications:

Published Applications

Read 10 publications using MAB612 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 2

The active form of TGF-beta 2 is a disulfide-linked homodimer which is generated by activation of the latent TGF-beta 2 complex. TGF-beta s are multifunctional cytokines that activate or repress multiple genes in a wide variety of cell types. Depending on the cell context, TGF-beta can control many cellular processes including growth, morphogenesis, and apoptosis.

Long Name

Transforming Growth Factor beta 2

Alternate Names

TGFB2, TGFbeta 2

Entrez Gene IDs

7042 (Human); 21808 (Mouse); 397084 (Porcine)

Gene Symbol

TGFB2

Additional TGF-beta 2 Products

Product Documents for TGF-beta 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for TGF-beta 2 Antibody

For research use only

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