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TFEB Antibody

Catalog # NB100-93447 | Novus Biologicals a Bio-Techne Brand
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NB100-93447
NB100-93447-20ug

Key Product Details

Species Reactivity

Human, Rat

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for TFEB Antibody

Immunogen

Peptide with sequence C-QKDLQKSRELENH corresponding to internal region according to NP_009093.1.

Epitope

C-QKDLQKSRELENH

Specificity

This antibody is expected not to cross-react to TFE3 and MITF.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TFEB Antibody

Western Blot: TFEB Antibody [NB100-93447] - Representative western blots of Atg7, TFEB and p62 at different times after LPS injection. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-08722-3), licensed under a CC-BY license.
Western Blot: TFEB Antibody [NB100-93447] - (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TFEB Antibody

Western Blot: TFEB Antibody [NB100-93447] -

LPS injection induces transient autophagy activation in rat hippocampus. (B) Representative western blots of Atg7, TFEB and p62 at different times after LPS injection. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-08722-3), licensed under a CC-BY licence.

Applications for TFEB Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human lung lysates (calculated MW of 52.9 kDa band according to NP_009093.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 2 publications using NB100-93447 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TFEB

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Long Name

Transcription Factor EB

Alternate Names

ALPHATFEB, BHLHE35, TCFEB

Entrez Gene IDs

7942 (Human); 21425 (Mouse); 316214 (Rat)

Gene Symbol

TFEB

UniProt

Product Documents for TFEB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TFEB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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