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TetR Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13057

Novus Biologicals, part of Bio-Techne
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NBP3-13057

Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Full length TetR recombinant protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for TetR Antibody - BSA Free

Western Blot: TetR Antibody [NBP3-13057]

Western Blot: TetR Antibody [NBP3-13057]

Western Blot: TetR Antibody [NBP3-13057] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TetR antibody (NBP3-13057) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
ELISA: TetR Antibody [NBP3-13057]

ELISA: TetR Antibody [NBP3-13057]

ELISA: TetR Antibody [NBP3-13057] - Indirect ELISA analysis performed by coating plate with recombinant full-length TetR (1000-15.63 ng/mL). Coated protein was probed with TetR antibody (NBP3-13057) (1 ug/mL). Rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.

Applications for TetR Antibody - BSA Free

Application
Recommended Usage

ELISA

Assay dependent

Western Blot

Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TetR

TetR, Tet repressor protein, is a 23kD protein that regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.

Alternate Names

pSC101 tetR, Tet Repressor, Tet Repressor Protein, TetR, tetracycline repressor protein

Additional TetR Products

Product Documents for TetR Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TetR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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