TET3 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20602
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Xenopus
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Cited:
Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chemotaxis, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.
Reactivity Notes
Use in Rat reported in scientific literature (PMID:34246869)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
179 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TET3 Antibody - Azide and BSA Free
![Western Blot: TET3 Antibody [NBP2-20602]](https://resources.bio-techne.com/images/products/TET3-Antibody-Western-Blot-NBP2-20602-img0013.jpg "Western Blot: TET3 Antibody [NBP2-20602]")
Western Blot: TET3 Antibody [NBP2-20602]
TET3-Antibody-Western-Blot-NBP2-20602-img0013.jpg![Immunoprecipitation: TET3 Antibody [NBP2-20602]](https://resources.bio-techne.com/images/products/TET3-Antibody-Immunoprecipitation-NBP2-20602-img0002.jpg "Immunoprecipitation: TET3 Antibody [NBP2-20602]")
Immunoprecipitation: TET3 Antibody [NBP2-20602]
Immunoprecipitation: TET3 Antibody [NBP2-20602] - Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 ug of TET3 antibody 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] -
Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] - BMMSCs express Tet1, Tet2, & Tet3. a, b Both human (h) & mouse (m) BMMSCs expressed Tet1, Tet2, & Tet3, as assessed by western blotting (a) & qPCR (b). c Immunocytofluorescent staining showed that CD146-positive BMMSCs expressed Tet1, Tet2, & Tet3. Scale bar, 50 μm. Results are from three independent experiments. ***p <0.001; p values calculated using two-tailed Student's t test (mean ± SD) Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-04464-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Applications for TET3 Antibody - Azide and BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Assay dependent
Flow Cytometry
Reported in scientific literature (PMID:34246869)
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry Whole-Mount
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:500-1:1000
Western Blot
1:1000-1:10000
Reviewed Applications
Read 1 review rated 4 using NBP2-20602 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TET3
Alternate Names
EC 1.14.11.n2, hCG_40738, KIAA0401, methylcytosine dioxygenase TET3, MGC22014, probable methylcytosine dioxygenase TET3, tet oncogene family member 3
Gene Symbol
TET3
UniProt
Additional TET3 Products
Product Documents for TET3 Antibody - Azide and BSA Free
Product Specific Notices for TET3 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.