Key Product Details

Species Reactivity

C. tetani

Applications

Western Blot, Immunoprecipitation

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 604023

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TeNT Light Chain Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant C. tetani TeNT Light Chain
Pro2-Gly430
Accession # P04958

Reactivity Notes

C. tetani

Specificity

Detects TeNT Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for TeNT Light Chain/Tetanus Toxin Antibody (604023) [DyLight 488]

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TeNT Light Chain

Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2. The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.

Long Name

Tetanus Neurotoxin Type Light Chain

Alternate Names

TeNT-LC, Tentoxylysin, tetX

Gene Symbol

TETX

Additional TeNT Light Chain Products

Product Documents for TeNT Light Chain/Tetanus Toxin Antibody (604023) [DyLight 488]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TeNT Light Chain/Tetanus Toxin Antibody (604023) [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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