Tenascin C Antibody (4C8MS) [Allophycocyanin/Cy7]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-68136APCCY7

Catalog #
Availability
Size / Price
Qty
NB110-68136APCCY7
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Conjugate
Catalog #
Alexa Fluor 350
NB110-68136AF350
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Alexa Fluor 405
NB110-68136AF405
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Alexa Fluor 488
NB110-68136AF488
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Alexa Fluor 532
NB110-68136AF532
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Alexa Fluor 594
NB110-68136AF594
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Alexa Fluor 647
NB110-68136AF647
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Alexa Fluor 700
NB110-68136AF700
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Alexa Fluor 750
NB110-68136AF750
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Alexa Fluor Plus 405
NB110-68136AFP405
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Alexa Fluor Plus 488
NB110-68136AFP488
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Alexa Fluor Plus 555
NB110-68136AFP555
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Alexa Fluor Plus 594
NB110-68136AFP594
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Alexa Fluor Plus 647
NB110-68136AFP647
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Alexa Fluor Plus 680
NB110-68136AFP680
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Allophycocyanin
NB110-68136APC
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Biotin
NB110-68136B
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CoraFluor 1
NB110-68136CL1
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DyLight 350
NB110-68136UV
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DyLight 405
NB110-68136V
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DyLight 488
NB110-68136G
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DyLight 550
NB110-68136R
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DyLight 594
NB110-68136DL594
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DyLight 650
NB110-68136C
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DyLight 680
NB110-68136FR
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DyLight 755
NB110-68136IR
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FITC
NB110-68136F
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HRP
NB110-68136H
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Janelia Fluor 525
NB110-68136JF525
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Janelia Fluor 549
NB110-68136JF549
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Janelia Fluor 646
NB110-68136JF646
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Janelia Fluor 669
NB110-68136JF669
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mFluor Violet 450 SE
NB110-68136MFV450
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mFluor Violet 500 SE
NB110-68136MFV500
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mFluor Violet 610 SE
NB110-68136MFV610
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PE
NB110-68136PE
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PE/Atto594
NB110-68136PEATT594
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PE/Cy5.5
NB110-68136PECY55
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PE/Cy7
NB110-68136PECY7
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PerCP
NB110-68136PCP
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Feline

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Allophycocyanin/Cy7 (Excitation = 650;755 nm, Emission = 767 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 4C8MS

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Tenascin C Antibodies »

Product Specifications

Immunogen

Recombinant human Tenascin C [Swiss-Prot# P24821].

Reactivity Notes

Feline reactivity reported in customer review.

Localization

Secreted > extracellular space > extracellular matrix

Specificity

NB110-68136 specifically reacts with Domain B on FNIII repeats of Tenascin C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for Tenascin C Antibody (4C8MS) [Allophycocyanin/Cy7]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

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Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Tenascin C

Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. Tenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen. Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).

Alternate Names

Cytotactin, HXB, Tenascin J1, TNC

Gene Symbol

TNC

Additional Tenascin C Products

Product Documents for Tenascin C Antibody (4C8MS) [Allophycocyanin/Cy7]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Tenascin C Antibody (4C8MS) [Allophycocyanin/Cy7]

Cy is a registered trademark of Global Life Sciences Solutions Germany GmbH.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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