TdT Antibody (MSVA-453R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57235
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # MSVA-453R
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human TdT protein Swissprot P04053
Localization
Nucleus
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.
Positive Control
Postive control:Thymus: At least a moderate nuclear immunostaining should be seen in virtually all cortical lymphocytes of the normal thymus. .
Negative Control
Negitve control:Thymus: The vast majority of the medullary lymphocytes of the normal thymus should be negative. ; Tonsil: The vast majority of lymphoid cells and all epithelial cells should be negative.
Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.
Applications for TdT Antibody (MSVA-453R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:75 1:150
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Preservative
<0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TdT
Long Name
Terminal Deoxynucleotidyl Transferase
Alternate Names
DNTT
Gene Symbol
DNTT
Additional TdT Products
Product Specific Notices for TdT Antibody (MSVA-453R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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