TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37720
![Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free [NBP2-37720]](https://resources.bio-techne.com/images/products/TCR-V-beta-5-Antibody-MEM-262-Azide-Free-Flow-Cytometry-NBP2-37720-img0002.jpg "Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free [NBP2-37720]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunoprecipitation, Functional, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # MEM-262
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human thymoma cell line HPB-ALL.
Specificity
The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
Endotoxin level is less than 0.01 EU/ug of the protein
Description
Novus Biologicals Mouse TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free (NB500-520) is a monoclonal antibody validated for use in WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free
Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free [NBP2-37720]
Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide Free [NBP2-37720] - Analysis (surface staining) of human peripheral blood cells with anti-human TCR Vbeta5.3 related (MEM-262) purified, GAM/APC.![Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free [NBP2-37720]](https://resources.bio-techne.com/images/products/TCR-V-beta-5-Antibody-MEM-262-Azide-Free-Flow-Cytometry-NBP2-37720-img0001.jpg "Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free [NBP2-37720]")
Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free [NBP2-37720]
Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide Free [NBP2-37720] - Human peripheral blood lymphocytes stained with MEM-262 FITC and anti-CD3 (UCHT1) APC.Applications for TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Western Blot: this antibody uiniquely recognizes denatured TCR beta chains.
Functional Application: this antibody activates T cells (V beta 5-related subset)
Functional Application: this antibody activates T cells (V beta 5-related subset)
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TCR V beta 5
Alternate Names
T cell receptor beta locus, T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster, T-cell receptor, beta cluster, TCRB, TRB
Gene Symbol
TRB
Additional TCR V beta 5 Products
Product Documents for TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free
Product Specific Notices for TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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