TCR V beta 5 Antibody (MEM-262) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-520PE

Novus Biologicals, part of Bio-Techne
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NB500-520PE
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-262

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TCR V beta 5 Antibodies »

Product Specifications

Immunogen

Human thymoma cell line HPB-ALL.

Specificity

The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TCR V beta 5 Antibody (MEM-262) [PE]

TCR V beta 5 Antibody (MEM-262) [PE]

TCR V beta 5 Antibody (MEM-262) [PE] [NB500-520PE] -

TCR V beta 5 Antibody (MEM-262) [PE] [NB500-520PE] - Vial of PE conjugated antibody. PE has two excitation maxima, 498 nm excited by the Blue laser (488 nm) and 565 nm excited by the Yellow-Green laser (561 nm). Both result in emission at 578 nm.

Applications for TCR V beta 5 Antibody (MEM-262) [PE]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TCR V beta 5

The V beta5 TCR is a variant of the TCR beta chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR V beta5 has been reported to recognize some staphylococcus bacterial superantigens. MEM-262 antibody reacts with a variant of the TCR beta chain which is expressed on HPB-ALL cells (known expressing TCR V beta5.3). But the subpopulation of peripheral blood T cells recognized by this antibody is reported larger than other anti-TCR V beta5.3 antibodies.

Alternate Names

T cell receptor beta locus, T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster, T-cell receptor, beta cluster, TCRB, TRB

Gene Symbol

TRB

Additional TCR V beta 5 Products

Product Documents for TCR V beta 5 Antibody (MEM-262) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TCR V beta 5 Antibody (MEM-262) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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