TCR beta Antibody (IARC307) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43545
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # IARC307
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Derived from normal B cells in peripheral blood of a Burkitt's lymphoma patient. Screened more than 60 culture supernatants from lymphoblastoid cell lines of normal origin, Epstein-Barr virus (EBV)-carrying Burkitt lymphomas, EBV-negative Burkitt lymphomas and EBV-converted sublines of original EBV-negative Burkitt lymphoma human B-cell lines for their capacity to bind the synthetic peptide autoantigens. One lymphoblastoid B-cell line was identified, IARC307, that produces IgM kappa antibodies which specifically react with the CDR1 peptide corresponding to human Vbeta8.1 gene sequence. (Uniprot# P01850; A0A5B9)
Specificity
This antibody is specific for Vbeta8.1 CDR1 sequence CKPISGHNSLFQWYRQT.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
Scientific Data Images for TCR beta Antibody (IARC307) - Chimeric - BSA Free
Immunocytochemistry /Immunofluorescence: TCR beta Antibody (IARC307) - Chimeric - BSA Free [NBP3-43545] -
Immunocytochemistry /Immunofluorescence: TCR beta Antibody (IARC307) - Chimeric - BSA Free [NBP3-43545] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips stained with NBP3-43545 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43545, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
Flow Cytometry: TCR beta Antibody (IARC307) - Chimeric - BSA Free [NBP3-43545] -
Flow Cytometry: TCR beta Antibody (IARC307) - Chimeric - BSA Free [NBP3-43545] - Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-43545 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for TCR beta Antibody (IARC307) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TCR beta
Alternate Names
T cell receptor beta chain, TCRB, TCRbeta, Tib
Gene Symbol
Tcrb
Additional TCR beta Products
Product Documents for TCR beta Antibody (IARC307) - Chimeric - BSA Free
Product Specific Notices for TCR beta Antibody (IARC307) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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