TCR beta Antibody (H57-597) [mFluor Violet 500 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20118MFV500
Recombinant Monoclonal Antibody
![TCR beta Antibody (H57-597) [mFluor Violet 500 SE]](https://resources.bio-techne.com/images/products/nbp3-20118mfv500_rabbit-tcr-beta-mab-h57-597-mfluor-violet-500-se-57202314425690.png "TCR beta Antibody (H57-597) [mFluor Violet 500 SE] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Lambda Clone # H57-597
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
Epitope
H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
Specificity
mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Lambda
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for TCR beta Antibody (H57-597) [mFluor Violet 500 SE]
TCR beta Antibody (H57-597) [mFluor Violet 500 SE] -
TCR beta Antibody (H57-597) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.Applications for TCR beta Antibody (H57-597) [mFluor Violet 500 SE]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP3-20118. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: TCR beta
Alternate Names
T cell receptor beta chain, TCRB, TCRbeta, Tib
Gene Symbol
Tcrb
Additional TCR beta Products
Product Documents for TCR beta Antibody (H57-597) [mFluor Violet 500 SE]
Product Specific Notices for TCR beta Antibody (H57-597) [mFluor Violet 500 SE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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