TCF20 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31968
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rat (90%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SEGSSQPEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEPPRLNASPAAREEATSPGAKDMPLSSDGNPKVNEKTVGVIVS
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TCF20 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: TCF20 Antibody [NBP2-31968]](https://resources.bio-techne.com/images/products/TCF20-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31968-img0007.jpg "Immunocytochemistry/ Immunofluorescence: TCF20 Antibody [NBP2-31968]")
Immunocytochemistry/ Immunofluorescence: TCF20 Antibody [NBP2-31968]
Immunocytochemistry/Immunofluorescence: TCF20 Antibody [NBP2-31968] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: TCF20 Antibody [NBP2-31968]](https://resources.bio-techne.com/images/products/TCF20-Antibody-Immunohistochemistry-NBP2-31968-img0004.jpg "Immunohistochemistry-Paraffin: TCF20 Antibody [NBP2-31968]")
Immunohistochemistry-Paraffin: TCF20 Antibody [NBP2-31968]
Immunohistochemistry-Paraffin: TCF20 Antibody [NBP2-31968] - Staining of human cerebellum shows distinct cytoplasmic and nuclear positivity in Purkinje cells.
Chromatin Immunoprecipitation ChIP: TCF20 Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-TCF20 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for TCF20 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TCF20
Alternate Names
AR1, SPBP, transcription factor 20, transcription factor 20 (AR1)
Gene Symbol
TCF20
UniProt
Additional TCF20 Products
Product Documents for TCF20 Antibody - BSA Free
Product Specific Notices for TCF20 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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