TCF-3/E2A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20585
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human TCF3. The exact sequence is proprietary.
Reactivity Notes
Rat (83%)
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit TCF-3/E2A Antibody - BSA Free (NBP2-20585) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TCF-3/E2A Antibody - BSA Free
![Western Blot: TCF-3/E2A Antibody [NBP2-20585]](https://resources.bio-techne.com/images/products/TCF3-E2A-Antibody-Western-Blot-NBP2-20585-img0001.jpg "Western Blot: TCF-3/E2A Antibody [NBP2-20585]")
Western Blot: TCF-3/E2A Antibody [NBP2-20585]
Western Blot: TCF3/E2A Antibody [NBP2-20585] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-20585] -
Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:600.Blue: Fluoroshield with DAPI .
Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-20585] -
Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for TCF-3/E2A Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Immunoprecipitation
1:100-1:500
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TCF-3/E2A
Long Name
Transcription Factor 3/E2-alpha
Alternate Names
bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR
Gene Symbol
TCF3
UniProt
Additional TCF-3/E2A Products
Product Documents for TCF-3/E2A Antibody - BSA Free
Product Specific Notices for TCF-3/E2A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.