TBC1D10A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36551
![Western Blot: TBC1D10A Antibody [NBP2-36551]](https://resources.bio-techne.com/images/products/TBC1D10A-Antibody-Western-Blot-NBP2-36551-img0001.jpg "Western Blot: TBC1D10A Antibody [NBP2-36551]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 458 to 508 of human EBP50-PDX Interactor of 64 kDa using the numbering given in entry NP_114143.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TBC1D10A Antibody - BSA Free
Western Blot: TBC1D10A Antibody [NBP2-36551]
Western Blot: TBC1D10A Antibody [NBP2-36551] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPI64 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: TBC1D10A Antibody [NBP2-36551]](https://resources.bio-techne.com/images/products/TBC1D10A-Antibody-Immunoprecipitation-NBP2-36551-img0002.jpg "Immunoprecipitation: TBC1D10A Antibody [NBP2-36551]")
Immunoprecipitation: TBC1D10A Antibody [NBP2-36551]
Immunoprecipitation: TBC1D10A Antibody [NBP2-36551] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated EPI64, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 secondsApplications for TBC1D10A Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
Reviewed Applications
Read 1 review rated 5 using NBP2-36551 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TBC1D10A
Alternate Names
EBP50-PDX interactor of 64 kDa, EPI64, rab27A-GAP-alpha, TBC1 domain family member 10A, TBC1 domain family, member 10A
Gene Symbol
TBC1D10A
UniProt
Additional TBC1D10A Products
Product Documents for TBC1D10A Antibody - BSA Free
Product Specific Notices for TBC1D10A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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