Syntenin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-53807

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NB100-53807
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Syntenin 1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence SLEDLKVDKVIQAQ-C corresponding to N-Terminus according to NP_001007068.1, NP_001007069, NP_001007070.1, NP_001007071.1.

Specificity

This antibody is expected to recognise isoform 1 (NP_005616.2 and NP_001007068.1) and isoform 3 (NP_001007070.1and NP_001007071.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Syntenin 1 Antibody

Western Blot: Syntenin 1 Antibody [NB100-53807]

Western Blot: Syntenin 1 Antibody [NB100-53807]

Western Blot: Syntenin 1 Antibody [NB100-53807] - Staining of Human HEK293 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NB100-53807]

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NB100-53807]

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NB100-53807] - Staining of paraffin embedded Human Breast. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Syntenin 1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.01 - 0.03 ug/mL
Application Notes
WB: Approx. 28-30 kDa band observed in HEK293 and A431 lysates (calculated MW of 32.4 kDa band according to NP_005616.2 and NP_001007068.1 and of 32.3 kDa band according to NP_001007070.1 and NP_001007071.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Syntenin 1

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

MDA9, MDA-9, melanoma differentiation associated protein-9, Melanoma differentiation-associated protein 9, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, Scaffold protein Pbp1, ST1, SYCLTACIP18, syndecan binding protein (syntenin), Syndecan-binding protein 1, syntenin-1

Entrez Gene IDs

6386 (Human); 53378 (Mouse); 83841 (Rat)

Gene Symbol

SDCBP

UniProt

NP_005616.2 (Human)

Additional Syntenin 1 Products

Product Documents for Syntenin 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Syntenin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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