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Syntenin 1 Antibody (23GB2185)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51022

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NBP3-51022-100ul
NBP3-51022-20ul

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2185

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human Syntenin 1 (Uniprot: O00560)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Syntenin 1 Antibody (23GB2185)

Syntenin 1 Antibody (23GB2185)

Immunocytochemistry/ Immunofluorescence: Syntenin 1 Antibody (23GB2185)[NBP3-51022] -

Syntenin 1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: Syndecan binding protein shRNA knockdown (KD). Nuclei were stained blue with DAPI; Syndecan binding protein was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Syntenin 1 Antibody (23GB2185)

Immunocytochemistry/ Immunofluorescence: Syntenin 1 Antibody (23GB2185)[NBP3-51022] -

Immunocytochemical staining of HepG2 cells with Syntenin 1 antibody(1:1,000) . Nuclei were stained blue with DAPI; Syntenin 1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Syntenin 1 Antibody (23GB2185)

Flow Cytometry: Syntenin 1 Antibody (23GB2185)[NBP3-51022] -

Validation of Syntenin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Syntenin 1 antibody (1:2,000) and analyzed using BD flow cytometer.

Applications for Syntenin 1 Antibody (23GB2185)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Syntenin 1

Syntenin 1 is encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

MDA9, MDA-9, melanoma differentiation associated protein-9, Melanoma differentiation-associated protein 9, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, Scaffold protein Pbp1, ST1, SYCLTACIP18, syndecan binding protein (syntenin), Syndecan-binding protein 1, syntenin-1

Gene Symbol

SDCBP

Additional Syntenin 1 Products

Product Documents for Syntenin 1 Antibody (23GB2185)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Syntenin 1 Antibody (23GB2185)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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