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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # V60P2C2*F2

Format

BSA Free

Concentration

1 mg/ml

Product Summary for Syndecan-4 Antibody (V60P2C2*F2) - BSA Free

Immunogen

Peptide Sequence - EDVVGPGQE

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Syndecan-4 Antibody (V60P2C2*F2) - BSA Free

Western Blot: Syndecan-4 Antibody (V60P2C2/F2) [NBP2-50315] - Analysis of Syndecan-4 in Hela cell lysate.

Applications for Syndecan-4 Antibody (V60P2C2*F2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): ELISA - Peptide immunogen; WB - Hela whole cell lysate. Product observed is larger than expected- could be due to post translational modifications as Syndecans are heavily glycosylated; IHC - formalin-fixed, paraffin-embedded multi tumour tissue microarray.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syndecan-4

Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

Alternate Names

Amphiglycan, Ryudocan, SDC4, Syndecan4

Gene Symbol

SDC4

Additional Syndecan-4 Products

Product Documents for Syndecan-4 Antibody (V60P2C2*F2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Syndecan-4 Antibody (V60P2C2*F2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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