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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 112

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Syndecan-4 Antibody (112)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Syndecan-4 (Uniprot#: O35988; Met1-Val146).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Syndecan-4 Antibody (112)

Western Blot: Syndecan-4 Antibody (112) [NBP2-90701] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa
Flow Cytometry: Syndecan-4 Antibody (112) [NBP2-90701] - Analysis of Mouse SDC4 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SDC4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for Syndecan-4 Antibody (112)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syndecan-4

Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

Alternate Names

Amphiglycan, Ryudocan, SDC4, Syndecan4

Gene Symbol

SDC4

Additional Syndecan-4 Products

Product Documents for Syndecan-4 Antibody (112)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Syndecan-4 Antibody (112)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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