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Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 047

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Syndecan-4 Antibody (047)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Syndecan-4 (Uniprot#: O35988; Met1-Val146).

Specificity

No cross-reactivity in ELISA with: Mouse SDC1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Syndecan-4 Antibody (047)

Immunocytochemistry/Immunofluorescence: Syndecan-4 Antibody (047) [NBP2-90700] - Immunofluorescence staining of SDC4 in Hela cells or mouse splenocytes. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse SDC4 monoclonal antibody (1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunohistochemistry-Paraffin: Syndecan-4 Antibody (047) [NBP2-90700] - Staining of mouse SDC4 in mouse intestine with rabbit monoclonal antibody (1:200).
Immunohistochemistry-Paraffin: Syndecan-4 Antibody (047) [NBP2-90700] - Staining of mouse SDC4 in mouse stomach with rabbit monoclonal antibody (1:200).

Applications for Syndecan-4 Antibody (047)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syndecan-4

Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

Alternate Names

Amphiglycan, Ryudocan, SDC4, Syndecan4

Gene Symbol

SDC4

Additional Syndecan-4 Products

Product Documents for Syndecan-4 Antibody (047)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Syndecan-4 Antibody (047)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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