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Synaptophysin Antibody

Catalog # NB300-653 | Novus Biologicals a Bio-Techne Brand
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NB300-653
NB300-653SS

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Feline, Monkey

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Synaptophysin Antibody

Immunogen

Synthetic peptide taken within amino acid region 1-100 on human Synaptophysin protein.

Reactivity Notes

Feline, Rat reactivity reported from a verified customer review.

Localization

Integral membrane protein. Synaptic vesicles.

Specificity

This antibody does not cross-react with other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens.

Marker

Pre-synaptic marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synaptophysin Antibody

Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.
Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653] - Feline retina. Synaptophysin (green), DAPI (blue). The image was capture by epifluorescent microscopy. Image from verified customer review.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.

Applications for Synaptophysin Antibody

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:125

Western Blot

1:500-1:1000
Application Notes
Immunofluorescence reactivity reported in (PMID: 21368026). Immunoprecipitation reactivity reported in (PMID: 22970294). Synaptophysin antibody validated for IP, IHC-Frozen from verified customer reviews.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB300-653 in the following applications:

Published Applications

Read 10 publications using NB300-653 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synaptophysin

Synaptophysin is a 38 kDa synaptic vesicle associated protein. The pathology of synaptic components is investigated by measuring the expression of synaptophysin that is mainly concentrated in synaptic terminals. The expression of synaptophysin is a measure of dynamic plastic changes in nerve tissue. A decrease in synaptophysin and other synaptic vesicle associated proteins (Synaptotagmin, synaptosomal associated protein 25 kDa, SNAP-25, CLC ad GAP-43) generally means degeneration in the nerve fibers and an increased expression results in enhanced neuroplasticity and neuronal hypertrophy. In conditions involving generative changes in the nerve tissue, the expression of psynaptophysin is generally reduced, for example, in Schizophrenia an Alzhiemers disease. A decreased expression of synaptophysin in hippocampus of Schizoprenic patients has also been reported.

Alternate Names

SYP

Entrez Gene IDs

6855 (Human); 20977 (Mouse); 24804 (Rat)

Gene Symbol

SYP

UniProt

Product Documents for Synaptophysin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Synaptophysin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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