Synapsin I [p Ser603] Antibody - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-181

Novus Biologicals, part of Bio-Techne
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NB300-181
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig

Cited:

Human, Guinea Pig

Predicted:

Bovine (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Synapsin I Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser603 conjugated to KLH. Accession # P17599

Reactivity Notes

Guinea Pig reactivity reported in (PMID: 29890692). Use in Human reported in secitific publication PMID: 32449987

Modification

p Ser603

Localization

Cell junction; synapse

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser603 . Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Marker

pre-Synaptic Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synapsin I [p Ser603] Antibody - Azide Free

Western Blot: Synapsin I [p Ser603] Antibody [NB300-181]

Western Blot: Synapsin I [p Ser603] Antibody [NB300-181]

Synapsin-I-[p-Ser603]-Antibody-Western-Blot-NB300-181-img0002.jpg
Western Blot: Synapsin I [p Ser603] Antibody [NB300-181]

Western Blot: Synapsin I [p Ser603] Antibody [NB300-181]

Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - Rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (l-Ptase, 1200 units for 30 minutes).
Synapsin I [p Ser603] Antibody

Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] -

Examples of Western blots of p-/synapsin 1 and p-/Ca2+-calmodulin-dependent kinase II. The figure depicts examples of the Western blots of the four synaptic plasticity markers investigated. The two bands seen in the p-synapsin and synapsin Western blots (70 and 74 kDa respectively) are consistent with splice variants as confirmed by manufacturer. P-CAMKII and CAMKII were detected around 50 kDa in accordance with previous findings.
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Applications for Synapsin I [p Ser603] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Synapsin I

Synapsin I (synapsin Ia and Ib, MW ~ 80,000 and 77,000 are collectively referred to as synapsin I) is a neuron specific protein that is localized to nerve terminals. The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis. It is thought that synapsin I cross-links synaptic vesicles to the cytoskeleton and thereby limits the ability of these synaptic vesicles to move to active zones and fuse with the plasma membrane during exocytosis. In addition to their role in neurotransmission, the synapsins are also thought to play a role in synapse formation. The appearance of synapsin I immunoreactivity correlates precisely with the development of synapses in the CNS. The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis. The appearance of synapsin I was a precise indicator of synapse formation and that synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis.

Alternate Names

SYN1, phospho Synapsin

Entrez Gene IDs

24949 (Rat)

Gene Symbol

SYN1

Additional Synapsin I Products

Product Documents for Synapsin I [p Ser603] Antibody - Azide Free

Product Datasheets

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Product Specific Notices for Synapsin I [p Ser603] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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