STRO-1 Antibody (STRO-1) - BSA Free

Immunocytochemistry/ Immunofluorescence: STRO-1 Antibody (STRO-1) [NBP1-48356]

Immunocytochemistry/Immunofluorescence: STRO-1 Antibody (STRO-1) [NBP1-48356] - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-STRO-1 (STRO-1) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/ Immunofluorescence: STRO-1 Antibody (STRO-1) - BSA Free [NBP1-48356] -

Characterization of SCAPs: (a) isolated SCAPs were positive for STRO-1 by immunocytochemistry; (b) isolated SCAPs were negative for CK by immunocytochemistry; (c) PBS served as a negative control; (d) flow cytometric analysis revealed that cultured SCAPs are positive for CD73 (99.99%), CD105 (99.99%), CD90 (99.94%), and CD146 (91.84%), but negative for CD34 (0.84%) and CD45 (2.97%). Mouse IgG isotype control antibodies conjugated to FITC, PE, APC, or PerCP were used as negative controls. Scale bars: 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24864235), licensed under a CC-BY license. Not internally tested by Novus Biologicals.