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STK39 Antibody

Catalog # NBP1-51955 | Novus Biologicals a Bio-Techne Brand
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NBP1-51955

Key Product Details

Species Reactivity

Human

Applications

Control, Immunohistochemistry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for STK39 Antibody

Immunogen

Peptide with sequence C-SQEKSRRVKEENPE corresponding to internal region according to NP_037365.2.

Epitope

C-SQEKSRRVKEENPE

Predicted Species

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for STK39 Antibody

Western Blot: STK39 Antibody [NBP1-51955] - Staining (0.3ug/ml) of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry: STK39 Antibody [NBP1-51955] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Control: STK39 Antibody [NBP1-51955] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

Applications for STK39 Antibody

Application
Recommended Usage

Immunohistochemistry

6.0 ug/ml

Peptide ELISA

Detection limit 1:4000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 65 kDa band observed in human brain (cerebellum) lysates and in Jurkat lysates (calculated MW of 59.5 kDa band according to NP_037365.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using NBP1-51955 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STK39

This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Alternate Names

DCHTsmall intestine SPAK-like kinase, DKFZp686K05124, EC 2.7.11, EC 2.7.11.1, PASK, proline-alanine-rich STE20-related kinase, serine threonine kinase 39, Serine/threonine-protein kinase 39, SPAKserine threonine kinase 39 (STE20/SPS1 homolog, yeast), STE20/SPS1 homolog, STE20/SPS1-related proline-alanine-rich protein kinase, Ste20-like protein kinase, Ste-20-related kinase

Entrez Gene IDs

27347 (Human); 53416 (Mouse); 54348 (Rat)

Gene Symbol

STK39

Product Documents for STK39 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for STK39 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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