STAT3 Antibody (OTI21E7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48014
![Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]](https://resources.bio-techne.com/images/products/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0003.jpg "Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI21E7
Concentration
0.35 mg/ml
Product Specifications
Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 154-175 of human stat3
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody is specific for Homo sapiens signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
88.08 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for STAT3 Antibody (OTI21E7)
In support of our commitment to scientific transparency, some data images have been temporarily removed from this page pending internal review. Contact us for more information.
Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] Staining of paraffin-embedded thyroid using anti-STAT3 mouse monoclonal antibody.
![Western Blot: STAT3 Antibody (OTI21E7) [NBP1-48014]](https://resources.bio-techne.com/images/products/STAT3-Antibody-21E7-Western-Blot-NBP1-48014-img0001.jpg "Western Blot: STAT3 Antibody (OTI21E7) [NBP1-48014]")
Western Blot: STAT3 Antibody (OTI21E7) [NBP1-48014]
Western Blot: STAT3 Antibody (21E7) [NBP1-48014] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT3(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3.![Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]](https://resources.bio-techne.com/images/products/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0002.jpg "Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]")
Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]
Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded breast using anti-STAT3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]](https://resources.bio-techne.com/images/products/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0004.jpg "Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]")
![Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]](https://resources.bio-techne.com/images/products/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0005.jpg "Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]")
![Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]](https://resources.bio-techne.com/images/products/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0006.jpg "Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]")
![Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]](https://resources.bio-techne.com/images/products/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0007.jpg "Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]")
![Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]](https://resources.bio-techne.com/images/products/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0008.jpg "Immunohistochemistry-Paraffin: STAT3 Antibody (OTI21E7) [NBP1-48014]")
Applications for STAT3 Antibody (OTI21E7)
Application
Recommended Usage
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.35 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: STAT3
Long Name
Signal Transducer and Activator of Transcription 3
Alternate Names
Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)
Entrez Gene IDs
6774 (Human)
Gene Symbol
STAT3
Additional STAT3 Products
Product Documents for STAT3 Antibody (OTI21E7)
Product Specific Notices for STAT3 Antibody (OTI21E7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...