Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PSM1

Format

BSA Free

Concentration

1.0 mg/ml

View all STAT1 Antibodies »

Product Specifications

Immunogen

STAT1 peptide sequence 721-733 (Ser727 phosphorylated).

Modification

p Ser727

Specificity

The antibody PSM1 recognizes transcriptional factor STAT1 (91 kDa) activated by phosphorylation at Ser727.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT1 [p Ser727] Antibody (PSM1) - BSA Free

Western Blot: STAT1 [p Ser727] Antibody (PSM1) [NB500-515] - Analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-a treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2) Lane 2: original whole cell lysate.

Applications for STAT1 [p Ser727] Antibody (PSM1) - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
WB: Detects a band of approximately 91 kDa. Purity is > 95% (by SDS-PAGE).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: STAT1

STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Entrez Gene IDs

6772 (Human)

Gene Symbol

STAT1

UniProt

P42224

Additional STAT1 Products

Product Documents for STAT1 [p Ser727] Antibody (PSM1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STAT1 [p Ser727] Antibody (PSM1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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