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ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32451

Novus Biologicals, part of Bio-Techne
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NBP1-32451

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Chicken (92%), Chimpanzee (99%), Mouse (96%), Rat (96%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (81%).

Localization

Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein, Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free (NBP1-32451) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images

ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free

Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody [NBP1-32451] -

K562 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 antibody [N2C3] (NBP1-32451) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody - BSA Free

Immunohistochemistry-Paraffin: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody [NBP1-32451] -

ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 antibody [N2C3] detects ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human cerebellum.
ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 stained by ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 antibody [N2C3] (NBP1-32451) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4A. [provided by RefSeq]

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 2

Alternate Names

Alpha 2,3-ST 2, Sialyltransferase 4B, SIAT4B, ST3GalA.2

Gene Symbol

ST3GAL2

Additional ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.