SPRED1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81927
![Western Blot: SPRED1 AntibodyBSA Free [NBP2-81927]](https://resources.bio-techne.com/images/products/SPRED1-Antibody-Western-Blot-NBP2-81927-img0001.jpg "Western Blot: SPRED1 AntibodyBSA Free [NBP2-81927]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
SPRED1 antibody was raised against a 14 amino acid synthetic peptide near the center of the human SPRED1. The immunogen is located within amino acids 250 - 300 of SPRED1. Amino Acid Squence: RHPDMWKNDLERDD
Specificity
This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
498 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit SPRED1 Antibody - BSA Free (NBP2-81927) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SPRED1 Antibody - BSA Free
Western Blot: SPRED1 AntibodyBSA Free [NBP2-81927]
Western Blot: SPRED1 Antibody [NBP2-81927] - Analysis of SPRED1 in human brain tissue lysate with SPRED1 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry: SPRED1 Antibody - BSA Free [NBP2-81927] -
Immunohistochemistry: SPRED1 Antibody - BSA Free [NBP2-81927] - Immunohistochemistry of SPRED1 in human brain tissue with SPRED1 antibody at 2.5 u/mL.
Immunocytochemistry/ Immunofluorescence: SPRED1 Antibody - BSA Free [NBP2-81927] -
Immunocytochemistry/ Immunofluorescence: SPRED1 Antibody - BSA Free [NBP2-81927] - Immunofluorescence of SPRED1 in Human Brain tissue with SPRED1 antibody at 20 ug/mL.Applications for SPRED1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2.5 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SPRED1
SPRED1 (Sprouty-related protein with an EVH1 domain-1) is a 55 kDa member of the SPRED family of regulatory molecules. It inhibits mitogenic signaling by blocking Ras activation of Raf, and disrupts actin stress fiber formation by binding TESK1. Human SPRED1 is 444 amino acids (aa) in length. It contains an N-terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 6 - 123), a central KBD domain that binds to the SCFR (aa 233 - 285), and a C-terminal Cys-rich/Sprouty-related domain that mediates homo- and heterodimerization with SPRED2, binding to TESK1, and undergoes palmitoylation for membrane localization (aa 334 - 442). There is one splice variant that shows a nine Lys substitution for aa 269 - 444.
Long Name
Sprouty-related with an EVH1 Domain Containing-1
Alternate Names
NFLS
Gene Symbol
SPRED1
Additional SPRED1 Products
Product Documents for SPRED1 Antibody - BSA Free
Product Specific Notices for SPRED1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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