SP4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31983
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NQATGQQQIIIDPSQGLVQLQNQPQQLELVTTQLAGNAWQLVASTPPASKENNVSQP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit SP4 Antibody - BSA Free (NBP2-31983) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SP4 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: SP4 Antibody [NBP2-31983]](https://resources.bio-techne.com/images/products/SP4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31983-img0006.jpg "Immunocytochemistry/ Immunofluorescence: SP4 Antibody [NBP2-31983]")
Immunocytochemistry/ Immunofluorescence: SP4 Antibody [NBP2-31983]
Immunocytochemistry/Immunofluorescence: SP4 Antibody [NBP2-31983] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.![Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-31983]](https://resources.bio-techne.com/images/products/SP4-Antibody-Immunohistochemistry-NBP2-31983-img0002.jpg "Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-31983]")
Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-31983]
Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-31983] - Staining of human lymph node shows distinct nuclear positivity in non-germinal center cells.![SP4 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: SP4 Antibody - BSA Free [NBP2-31983]](https://resources.bio-techne.com/images/products/nbp2-31983_rabbit-polyclonal-sp4-antibody-25620259121929.jpg "Chromatin Immunoprecipitation-exo-Seq: SP4 Antibody - BSA Free [NBP2-31983]")
Chromatin Immunoprecipitation-exo-Seq: SP4 Antibody - BSA Free [NBP2-31983]
ChIP-Exo-Seq composite graph for Anti-SP4 (NBP2-31983) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for SP4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SP4
Alternate Names
MGC130008, MGC130009, Sp4 transcription factor, SPR-1HF1B, transcription factor Sp4
Gene Symbol
SP4
UniProt
Additional SP4 Products
Product Documents for SP4 Antibody - BSA Free
Product Specific Notices for SP4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...