SOX11 Antibody (CL0142) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43690

Novus Biologicals, part of Bio-Techne
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NBP3-43690
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BSA Free
NBP2-14782
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0142

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SOX11 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P48431, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Expected to cross react based on sequence identity: Rat (82%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SOX11 Antibody (CL0142) - Azide and BSA Free

SOX11 Antibody (CL0142) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]

Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]

Staining of eye in mouse embryo E14 retina shows moderate nuclear positivity in developing retina.
SOX11 Antibody (CL0142) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]

Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]

Staining of human chronic lymphocytic leukemia shows no nuclear positivity in tumor cells as expected.
SOX11 Antibody (CL0142) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]

Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]

Staining of human mantle cell lymphoma shows moderate to strong nuclear positivity in tumor cells.
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Applications for SOX11 Antibody (CL0142) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.

Background: SOX11

SOX11 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis.

Long Name

Transcription Factor SRY (Sex Determining Region Y)-box 11

Alternate Names

SRY (sex determining region Y)-box 11, SRY (sex-determining region Y)-box 11, SRY-related HMG-box gene 11, transcription factor SOX-11

Gene Symbol

SOX11

Additional SOX11 Products

Product Documents for SOX11 Antibody (CL0142) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOX11 Antibody (CL0142) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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