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SORBS1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86641

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NBP1-86641
NBP1-86641-25ul

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DSSPLLNEVSSSLIGTDSQAFPSVSKPSSAYPSTTIVNPTIVLLQHNREQQKRLSSLSDPVSERRVGEQDSAPTQEKPTSPGKAIEKRAKDDSRRVVKSTQDLSDVSMDEVGIPLRNTERSKDWYKTMFKQIHKLNR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SORBS1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SORBS1 Antibody [NBP1-86641]

Immunocytochemistry/ Immunofluorescence: SORBS1 Antibody [NBP1-86641]

Immunocytochemistry/Immunofluorescence: SORBS1 Antibody [NBP1-86641] - Staining of human cell line U-2 OS shows localization to plasma membrane, centrosome & focal adhesion sites. Antibody staining is shown in green.
SORBS1 Antibody - BSA Free Western Blot: SORBS1 Antibody - BSA Free [NBP1-86641]

Western Blot: SORBS1 Antibody - BSA Free [NBP1-86641]

Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SORBS1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
SORBS1 Antibody - BSA Free Immunohistochemistry-Paraffin: SORBS1 Antibody - BSA Free [NBP1-86641]

Immunohistochemistry-Paraffin: SORBS1 Antibody - BSA Free [NBP1-86641]

Staining of human endometrium, gastrointestinal, heart muscle and testis using Anti-SORBS1 antibody NBP1-86641 (A) shows similar protein distribution across tissues to independent antibody NBP2-33383 (B).

Applications for SORBS1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SORBS1

SORBS1 plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions

Alternate Names

c-Cbl associated protein, c-Cbl-associated protein, DKFZp451C066, DKFZp586P1422, Fas-ligand associated factor 2, FLAF2, KIAA0894, KIAA1296CAPSH3D5SH3P12FLJ12406, ponsin, R85FL, SH3 domain protein 5, SH3-domain protein 5 (ponsin), sh3p12, SORB1, sorbin and SH3 domain containing 1, sorbin and SH3 domain-containing protein 1

Gene Symbol

SORBS1

Additional SORBS1 Products

Product Documents for SORBS1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SORBS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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