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Somatostatin R2/SSTR2 Antibody

Catalog # NB300-157 | Novus Biologicals a Bio-Techne Brand
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NB300-157
NB300-157SS

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine

Applications

Electron Microscopy, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Summary for Somatostatin R2/SSTR2 Antibody

Immunogen

A synthetic peptide made to the C-terminus of rat Somatostatin Receptor 2 [UniProt# P30680]

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID:33002813)

Localization

Cell Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Somatostatin R2/SSTR2 Antibody

Immunocytochemistry/Immunofluorescence: Somatostatin R2/SSTR2 Antibody [NB300-157] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 1:50 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of Somatostatin Receptor 2 in mouse pancreas using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: Somatostatin R2/SSTR2 Antibody [NB300-157] - Staining of paraffin embedded rat pancreas tissue.

Applications for Somatostatin R2/SSTR2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:250

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 10 publications using NB300-157 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Somatostatin R2/SSTR2

Somatostatin is a tetradecapeptide that is widely distributed in the body. It acts on multiple organs in the body and also functions as a neuropeptide affecting electrical activity of neurons. Somatostatin Receptor 2 (SSR2), along with SSR1, is expressed at the highest levels in the stomach and jejunum, cerebrum and kidney, respectively.

Long Name

Somatostatin Receptor Type 2

Alternate Names

Smstr2, SomatostatinR2, SST2, SSTR2

Entrez Gene IDs

6752 (Human); 20606 (Mouse); 54305 (Rat)

Gene Symbol

SSTR2

Product Documents for Somatostatin R2/SSTR2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Somatostatin R2/SSTR2 Antibody

Licensed to Novus Biologicals LLC under U.S. Patent No 5,998,154.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for Somatostatin R2/SSTR2 Antibody (NB300-157):

Somatostatin R2/SSTR2 Antibody: https://www.novusbio.com/products/somatostatin-r2-sstr2-antibody_nb300-…
Procedure Guide for NB 300-157 Polyclonal Anti-Somatostatin Receptor II

Immunofluorescence Procedure

1. Plate cells on Lab-TekII chamber slides (Nalge Nunc).
2. Grow cells to about 75% confluence.
3. Rinse cells with PBS, at room temperature (RT).
4. Fix in 2% formaldehyde/PBS for 20 minutes at RT.
5. Rinse the cells 3x with TBS (pH 7.2).
6. Block the non-specific sites with 0.5% normal goat serum in TBS + 0.1% Triton X 100 (TBS-TX).
7. Incubate the cells in anti-SSR2 (catalog# NB 300-157), diluted in TBS-TX, overnight at RT.
8. Next day: rinse the cells 3x 5 minutes, in TBS.
9. Incubate the cells for 1 hour at RT in FITC-goat anti-rabbit secondary, diluted in TBS-TX (1:1,000).
10. Rinse 3x with TBS.
11. Mount the cells in Vector Labs mounting media and coverslip.

FAQs for Somatostatin R2/SSTR2 Antibody

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  • Q: Is there any data to show that this antibody specifically detects sstr2 since it says that it is unpurified sera?

    A: All of our current data for NB300-157 is available online. We have 6 available publications for this antibody as well. This product has been shown to specifically target the protein of interest by WB in published data. If you are interested in a blocking peptide to assist in your research, we do offer one specifically for this antibody - Somatostatin R2 SSTR2 Peptide (NB300-157PEP).

Showing  1 - 1 of 1 FAQ Showing All
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