SMAD6 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56440
![Western Blot: SMAD6 Antibody [NB100-56440]](https://resources.bio-techne.com/images/products/SMAD6-Antibody-Western-Blot-NB100-56440-img0002.jpg "Western Blot: SMAD6 Antibody [NB100-56440]")
![Simple Western: SMAD6 Antibody [NB100-56440]](https://resources.bio-techne.com/images/products/SMAD6-Antibody-Simple-Western-NB100-56440-img0003.jpg "Simple Western: SMAD6 Antibody [NB100-56440]")
![Western Blot: SMAD6 Antibody [NB100-56440]](https://resources.bio-techne.com/images/products/SMAD6-Antibody-Western-Blot-NB100-56440-img0006.jpg "Western Blot: SMAD6 Antibody [NB100-56440]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey, Primate, Sheep
Cited:
Human, Mouse, Rat, Ovine
Predicted:
Equine (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Synthetic peptide made to an internal portion of human SMAD6 (between amino acids 30-150) [UniProt O43541].
Reactivity Notes
Cross reacts with New World Monkey. Predicted to react with Equine. Mouse reactivity reported from a customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SMAD6 Antibody - BSA Free
Western Blot: SMAD6 Antibody [NB100-56440]
Western Blot: SMAD6 Antibody [NB100-56440] - Analysis in mouse adult lung tissue. WB image submitted by a verified customer review.![Immunocytochemistry/ Immunofluorescence: SMAD6 Antibody [NB100-56440]](https://resources.bio-techne.com/images/products/SMAD6-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56440-img0004.jpg "Immunocytochemistry/ Immunofluorescence: SMAD6 Antibody [NB100-56440]")
Immunocytochemistry/ Immunofluorescence: SMAD6 Antibody [NB100-56440]
Immunocytochemistry/Immunofluorescence: SMAD6 Antibody [NB100-56440] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-SMAD6 (NB100-56440) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: SMAD6 Antibody [NB100-56440]](https://resources.bio-techne.com/images/products/SMAD6-Antibody-Immunohistochemistry-Paraffin-NB100-56440-img0005.jpg "Immunohistochemistry-Paraffin: SMAD6 Antibody [NB100-56440]")
Immunohistochemistry-Paraffin: SMAD6 Antibody [NB100-56440]
Immunohistochemistry-Paraffin: SMAD6 Antibody [NB100-56440] - SMAD6 was detected in immersion fixed paraffin-embedded sections of human lung using Rabbit Anti-Human SMAD6 polyclonal Antibody (Catalog # NB100-56440) at 5 ug/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Nuclear staining was localized to the pneumocytes.Applications for SMAD6 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 - 5 ug/mL. Use reported in scientific literature (PMID 20032053)
Immunohistochemistry
5 - 10 ug/mL
Immunohistochemistry-Paraffin
5 - 10 ug/mL. Use reported in scientific literature (PMID 20032053)
Simple Western
1:50
Western Blot
0.5 - 2 ug/mL. Use reported by customer review
Application Notes
A 52 kDa band is observed. Use of this antibody in IHC-P and ICC-IF applications is cited by Hogg et al 2010 in Endocrinology 151:1247-56 (PMID: 20032053) and this publication has documented protocols for these applications.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 54 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 54 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 reviews rated 5 using NB100-56440 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SMAD6
Alternate Names
hSMAD6, HsT17432, MAD homolog 6, MADH6SMAD, mothers against DPP homolog 6 (Drosophila), MADH7, mothers against decapentaplegic homolog 6, Mothers against decapentaplegic, drosophila, homolog of, 6, Mothers against DPP homolog 6, SMAD 6, SMAD family member 6MAD, mothers against decapentaplegic homolog 6 (Drosophila), SMAD, mothers against DPP homolog 6, Smad6
Gene Symbol
SMAD6
UniProt
Additional SMAD6 Products
Product Documents for SMAD6 Antibody - BSA Free
Product Specific Notices for SMAD6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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