SKI Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77305

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NBP1-77305
NBP1-77305-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chicken (100%), Equine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SKI Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski. The immunogen is located within the first 50 amino acids of Ski.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SKI Antibody - BSA Free

Western Blot: SKI AntibodyBSA Free [NBP1-77305]

Western Blot: SKI AntibodyBSA Free [NBP1-77305]

Western Blot: SKI Antibody [NBP1-77305] - Analysis in HeLa cell lysate with antibody at (A) 1 and (B) 2 ug/mL.
SKI Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SKI Antibody - BSA Free [NBP1-77305] -

Immunocytochemistry/ Immunofluorescence: SKI Antibody - BSA Free [NBP1-77305] - Immunofluorescence of SKI in human kidney tissue with SKI antibody at 20 ug/mL.

Applications for SKI Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Western Blot

1-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SKI

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.

Long Name

SKI Oncogene

Alternate Names

SKV

Gene Symbol

SKI

UniProt

P12755

Additional SKI Products

Product Documents for SKI Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SKI Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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