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SIT1 Antibody (SIT-01) - BSA Free

Catalog # NB500-486 | Novus Biologicals a Bio-Techne Brand

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SIT-01

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for SIT1 Antibody (SIT-01) - BSA Free

Immunogen

Bacterially produced recombinant intracellular fragment of human SIT.

Reactivity Notes

Weak cross-reactivity with mouse.

Specificity

The antibody SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SIT1 Antibody (SIT-01) - BSA Free

Western Blot: SIT1 Antibody (SIT-01) [NB500-486] - Analysis of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01 and goat anti-mouse IgG (H+L)-HRP conjugate.
Flow Cytometry: SIT1 Antibody (SIT-01) [NB500-486] - Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.

Applications for SIT1 Antibody (SIT-01) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-5 ug/ml

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SIT1

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.

Long Name

Signaling Threshold Regulating Transmembrane Adaptor 1

Alternate Names

gp30/40

Entrez Gene IDs

54390 (Mouse)

Gene Symbol

SIT1

Product Documents for SIT1 Antibody (SIT-01) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SIT1 Antibody (SIT-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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