SHARPIN Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04116
![Immunoprecipitation: SHARPIN Antibody [NBP2-04116]](https://resources.bio-techne.com/images/products/SHARPIN-Antibody-Immunoprecipitation-NBP2-04116-img0001.jpg "Immunoprecipitation: SHARPIN Antibody [NBP2-04116]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human Shank-Associated RH Domain-Interacting Protein using the numbering given in entry NP_112236.3 (GeneID 81858).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID:34755089).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SHARPIN Antibody - BSA Free
Immunoprecipitation: SHARPIN Antibody [NBP2-04116]
Immunoprecipitation: SHARPIN Antibody [NBP2-04116] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SHARPIN antibody NBP2-04116 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SHARPIN was also immunoprecipitated by rabbit anti-SHARPIN antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for SHARPIN Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reactivity reported in scientific literature (PMID:34755089).
Immunohistochemistry
Reactivity reported in scientific literature (PMID:34755089).
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SHARPIN
Long Name
SHANK-associated RH Domain Interactor
Alternate Names
SIPL1
Gene Symbol
SHARPIN
UniProt
Additional SHARPIN Products
Product Documents for SHARPIN Antibody - BSA Free
Product Specific Notices for SHARPIN Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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