Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all SFRS18 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 200 to 250 of human splicing factor, arginine/serine-rich 18 (splicing factor, arginine/serine-rich 130) using the numbering given in entry NP_056306.1 (GeneID 25957).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SFRS18 Antibody - BSA Free

Immunoprecipitation: SFRS18 Antibody [NB100-79851]

Immunoprecipitation: SFRS18 Antibody [NB100-79851]

Immunoprecipitation: SFRS18 Antibody [NB100-79851] - Detection of Human SFRS18/SRrp130 by Western Blot of Immunoprecipitates. For blSamples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SFRS18/SRrp130 antibody NB100-79851 used for IP at 3 mcg/mg lysate. SFRS18/SRrp130 was also immunoprecipitated by rabbit anti-SFRS18/SRrp130 antibody NB100-79852, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for SFRS18 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Western Blot Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SFRS18

SFRS18/SRrp130 (splicing factor arginine/serine-rich 18) is a putative alternative splicing factor. It was identified as a factor that interacts with pinin (Pnn/DRS/memA), a cell-adhesion and nuclear protein of corneal epithelial cells. SFRS18/SRrp130 bears multiple arginine/serine-rich repeats found among the SR-related family of pre-mRNA processing factors. SFRS18/SRrp130 and pinin co-localize and are distributed as nuclear specks that correspond to the pre-splicing machinery.

Alternate Names

bA98I9.2, C6orf111, chromosome 6 open reading frame 111, DKFZp564B0769, FLJ14752, FLJ14992, FLJ90147, HSPC306, MGC104269, RP11-98I9.2, Serine/arginine-rich-splicing regulatory protein 130, serine-arginine-rich-splicing regulatory protein 130, Splicing factor, arginine/serine-rich 130, splicing factor, arginine/serine-rich 18, SR rich protein, SR-related protein, SR-rich protein, SRRP130, SRrp130FLJ14853

Entrez Gene IDs

25957 (Human)

Gene Symbol

PNISR

UniProt

NP_056306.1 (Human)

Additional SFRS18 Products

Product Documents for SFRS18 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SFRS18 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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