sFRP-4 Antibody (7Z6Q1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16352
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7Z6Q1 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 201-300 of human sFRP-4 (Q6FHJ7). AKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit sFRP-4 Antibody (7Z6Q1) (NBP3-16352) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for sFRP-4 Antibody (7Z6Q1)
![Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]](https://resources.bio-techne.com/images/products/sFRP-4-Antibody-7Z6Q1-Immunocytochemistry-Immunofluorescence-NBP3-16352-img0002.jpg "Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]")
Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]
Immunocytochemistry/Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunofluorescence analysis of NIH-3T3 cells using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]](https://resources.bio-techne.com/images/products/sFRP-4-Antibody-7Z6Q1-Immunohistochemistry-Paraffin-NBP3-16352-img0001.jpg "Immunohistochemistry-Paraffin: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]")
Immunohistochemistry-Paraffin: sFRP-4 Antibody (7Z6Q1) [NBP3-16352]
Immunohistochemistry-Paraffin: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunohistochemistry of paraffin-embedded human colon using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] -
Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Confocal imaging of U-2 OS cells using sFRP-4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.Applications for sFRP-4 Antibody (7Z6Q1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: sFRP-4
Long Name
Secreted Frizzled Related Protein 4
Alternate Names
DDC-4, FRP-AP, frpHE, FrzB2, sFRP4
Gene Symbol
SFRP4
Additional sFRP-4 Products
Product Documents for sFRP-4 Antibody (7Z6Q1)
Product Specific Notices for sFRP-4 Antibody (7Z6Q1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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