SETDB1 Antibody (HL3260)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48557
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL3260
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human SETDB1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit SETDB1 Antibody (HL3260) (NBP3-48557) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SETDB1 Antibody (HL3260)
Western Blot: SETDB1 Antibody (HL3260) [NBP3-48557] -
Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3260] (NBP3-48557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody (HL3260) [NBP3-48557] -
SETDB1 antibody [HL3260] detects SETDB1 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SETDB1 stained by SETDB1 antibody [HL3260] (NBP3-48557) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Western Blot: SETDB1 Antibody (HL3260) [NBP3-48557] -
HeLa whole cell and nuclear extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3260] (NBP3-48557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for SETDB1 Antibody (HL3260)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SETDB1
Alternate Names
ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1
Gene Symbol
SETDB1
Additional SETDB1 Products
Product Documents for SETDB1 Antibody (HL3260)
Product Specific Notices for SETDB1 Antibody (HL3260)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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