SERPINB1 Antibody (05) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05868
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 05
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SERPINB1 (Uniprot#: P30740; Met1-Pro379).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for SERPINB1 Antibody (05) - Azide and BSA Free
Immunoprecipitation: SERPINB1 Antibody (05) [NBP3-05868] -
Immunoprecipitation: SERPINB1 Antibody (05) [NBP3-05868] - SERPINB1 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 0.5 uL NBP3-05868 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-05868, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size: 43 kDa
Western Blot: SERPINB1 Antibody (05) [NBP3-05868] -
Western Blot: SERPINB1 Antibody (05) [NBP3-05868] - NBP3-05868 at 1:500 dilution Lane A: NCI-H460 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDaApplications for SERPINB1 Antibody (05) - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
1-2 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SERPINB1
Alternate Names
EILEI, ELANH2MNEI, elastase, leukocyte elastase inhibitor, Monocyte/neutrophil elastase inhibitor, Peptidase inhibitor 2, PI-2, PI2M/NEI, protease inhibitor 2 (elastase), monocyte/neutrophil derived, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1, Serpin B1, serpin peptidase inhibitor, clade B (ovalbumin), member 1
Gene Symbol
SERPINB1
Additional SERPINB1 Products
Product Documents for SERPINB1 Antibody (05) - Azide and BSA Free
Product Specific Notices for SERPINB1 Antibody (05) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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