SERCA2 ATPase Antibody (23GB3250)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51099
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB3250
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human SERCA2 ATPase (Uniprot: P16615)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SERCA2 ATPase Antibody (23GB3250)
Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (23GB3250)[NBP3-51099] -
SERCA2 ATPase antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: ATP2A2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP2A2 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
Flow Cytometry: SERCA2 ATPase Antibody (23GB3250)[NBP3-51099] -
Flow cytometric analysis of SERCA2 ATPaseexpression in HeLa cells using SERCA2 ATPase antibody (1:2,000). Green, isotype control; red, ATP2A2.
Western Blot: SERCA2 ATPase Antibody (23GB3250)[NBP3-51099] -
Western blotting analysis using SERCA2 ATPase antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for SERCA2 ATPase Antibody (23GB3250)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SERCA2 ATPase
Long Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Alternate Names
ATP2A2, ATP2B, Calcium pump 2, SERCA2, SR Ca(2+)-ATPase 2
Gene Symbol
ATP2A2
Additional SERCA2 ATPase Products
Product Documents for SERCA2 ATPase Antibody (23GB3250)
Product Specific Notices for SERCA2 ATPase Antibody (23GB3250)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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