Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all SEC3 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 100 and 150 of human Exocyst Complex Component Sec3 using the numbering given in entry NP_060731.2 (GeneID 55763).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SEC3 Antibody - BSA Free

Immunoprecipitation: SEC3 Antibody [NBP1-78752]

Immunoprecipitation: SEC3 Antibody [NBP1-78752]

Immunoprecipitation: SEC3 Antibody [NBP1-78752] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Sec3 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Sec3 was also immunoprecipitated by rabbit anti-Sec3 antibody NBP1-78753 which recognizes a downstream epitope.

Applications for SEC3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SEC3

The EXOC1 (also known as SEC3) gene encodes an exocyst complex component 1 protein, that in isoform 1 is 894 amino acids long and nearly 102 kDA and in isoform 2 is 879 amino acids long and 100 kDA. The EXOC1 gene functions in the docking of exocytic vesicles with fusion sites on the plasma membrane. EXOC1 participates in the insulin pathway, Arf6 trafficking events, exocyst complex formation, and the metabolism of proteins through interacts with genes EXOC4, PKP3, LUC7L2, CDC5L, and C7orf55-LUC7L2. EXOC1 has been linked to toxic shock syndrome, schizophrenia, and mucopolysaccharidosis.

Alternate Names

BM-102, exocyst complex component 1, Exocyst complex component Sec3, FLJ10893, SEC3L1, SEC3-like 1, SEC3-like 1 (S. cerevisiae), SEC3P, SEC3Sec3p

Gene Symbol

EXOC1

UniProt

NP_060731.2 (Human)

Additional SEC3 Products

Product Documents for SEC3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SEC3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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