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SARS-CoV NSP8 Antibody (5A10), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP3-13528

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NBP3-13528

Key Product Details

Species Reactivity

Validated:

SARS-CoV, SARS-CoV-2

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5A10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for SARS-CoV NSP8 Antibody (5A10)

Immunogen

The immunogen used to generate this antibody corresponds to SARS virus nsp8.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SARS-CoV NSP8 Antibody (5A10)

Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - Non-infected (-) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:1000. Blue: Fluoroshield with DAPI.
Flow Cytometry: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis. Sample: Vero E6 cells infected with SARS-CoV-2. Black: Uninfected Vero E6 cells was used as a control. Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) dilution: 1:100.

Applications for SARS-CoV NSP8 Antibody (5A10)

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry-Frozen

Assay dependent

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NSP8

Long Name

Non-Structural Protein 8

Alternate Names

COVID-19

Additional NSP8 Products

Product Documents for SARS-CoV NSP8 Antibody (5A10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS-CoV NSP8 Antibody (5A10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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