SARS-CoV-2 nsp3 Antibody (HL2350) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25662
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
SARS-CoV-2
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2350 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the PL-pro domain of SARS-CoV-2 nsp3. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS-CoV-2 nsp3 Antibody (HL2350) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp3 Antibody (HL2350) - Azide and BSA Free [NBP3-25662] -
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp3 Antibody (HL2350) - Azide and BSA Free [NBP3-25662] - SARS-CoV-2 (COVID-19) nsp3 / PL-pro antibody [HL2350] detects SARS-CoV-2 (COVID-19) nsp3 / PL-pro protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp3 / PL-pro stained by SARS-CoV-2 (COVID-19) nsp3 / PL-pro antibody [HL2350] (NBP3-25662) diluted at 1:500. Blue: Fluoroshield with DAPI .
Western Blot: SARS-CoV-2 nsp3 Antibody (HL2350) - Azide and BSA Free [NBP3-25662] -
Western Blot: SARS-CoV-2 nsp3 Antibody (HL2350) - Azide and BSA Free [NBP3-25662] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp3 / PL-pro antibody [HL2350] (NBP3-25662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for SARS-CoV-2 nsp3 Antibody (HL2350) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 nsp3
Alternate Names
Non-structural protein 3, Papain-like protease, Papain-like proteinase, PL2-PRO, PL-PRO, SARS-CoV-2NSP3
Gene Symbol
ORF1ab
Additional SARS-CoV-2 nsp3 Products
Product Documents for SARS-CoV-2 nsp3 Antibody (HL2350) - Azide and BSA Free
Product Specific Notices for SARS-CoV-2 nsp3 Antibody (HL2350) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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