SARS-CoV-2 nsp2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13469

Novus Biologicals, part of Bio-Techne
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NBP3-13469
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SARS-CoV-2 nsp2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 nsp2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SARS-CoV-2 nsp2 Antibody - BSA Free

Western Blot: SARS-CoV-2 nsp2 Antibody [NBP3-13469]

Western Blot: SARS-CoV-2 nsp2 Antibody [NBP3-13469]

Western Blot: SARS-CoV-2 nsp2 Antibody [NBP3-13469] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp2 antibody (NBP3-13469) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 nsp2 Antibody [NBP3-13469]

Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 nsp2 Antibody [NBP3-13469]

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp2 Antibody [NBP3-13469] - SARS-CoV-2 (COVID-19) nsp2 antibody detects SARS-CoV-2 (COVID-19) nsp2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp2 stained by SARS-CoV-2 (COVID-19) nsp2 antibody (NBP3-13469) diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 nsp2 Antibody [NBP3-13469]

Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 nsp2 Antibody [NBP3-13469]

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp2 Antibody [NBP3-13469] - SARS-CoV-2 (COVID-19) nsp2 antibody detects SARS-CoV-2 (COVID-19) nsp2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp2 stained by SARS-CoV-2 (COVID-19) nsp2 antibody (NBP3-13469) diluted at 1:2000.

Applications for SARS-CoV-2 nsp2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000
Application Notes
ICC/IF-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp2

SARS-CoV-2 Nonstructural Protein 2 (NSP2) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP2 is 638 amino acids (aa) with a theoretical molecular weight of 70.5 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP2 has 68.3% sequence identity and 90% sequence similarity (1). SARS-CoV-2 NSP2 is shown to bind two host proteins, prohibitin 1 (PHB1) and prohibitin 2 (PHB2) (1). The NSP2-PHB protein interaction indicates that SARS-CoV-2 NBP2 might function in disturbing the host-cell environment during viral infection (1). A SARS-CoV-2 interactome study revealed that NSP2 has a role in vesicle trafficking, specifically reconfiguring endoplasmic reticulum and Golgi trafficking during viral infection via the WASH complex (Wiskott Aldrich Syndrome protein and scar homologue complex) (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

Alternate Names

Non-structural protein 2, NSP2, p65 homolog

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp2 Products

Product Documents for SARS-CoV-2 nsp2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS-CoV-2 nsp2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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