SARS-CoV-2 NSP10 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13475
Key Product Details
Species Reactivity
SARS-CoV-2
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full length SARS-CoV-2 NSP10 recombinant protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for SARS-CoV-2 NSP10 Antibody - BSA Free
![Western Blot: SARS-CoV-2 NSP10 Antibody [NBP3-13475]](https://resources.bio-techne.com/images/products/SARS-CoV-2-NSP10-Antibody-Western-Blot-NBP3-13475-img0001.jpg "Western Blot: SARS-CoV-2 NSP10 Antibody [NBP3-13475]")
Western Blot: SARS-CoV-2 NSP10 Antibody [NBP3-13475]
Western Blot: SARS-CoV-2 NSP10 Antibody [NBP3-13475] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp10 antibody (NBP3-13475) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody![Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 NSP10 Antibody [NBP3-13475]](https://resources.bio-techne.com/images/products/SARS-CoV-2-NSP10-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13475-img0003.jpg "Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 NSP10 Antibody [NBP3-13475]")
Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 NSP10 Antibody [NBP3-13475]
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 NSP10 Antibody [NBP3-13475] - SARS-CoV-2 (COVID-19) nsp10 antibody detects SARS-CoV-2 (COVID-19) nsp10 protein at cytoplasm by immunohistochemical analysis. Sample: Mock and SARS-CoV-2 (COVID-19) nsp10 transfected 293T cell FFPE Cell Pellet Block. Green: SARS-CoV-2 (COVID-19) nsp10 stained by SARS-CoV-2 (COVID-19) nsp10 antibody (NBP3-13475) diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 NSP10 Antibody [NBP3-13475]](https://resources.bio-techne.com/images/products/SARS-CoV-2-NSP10-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13475-img0002.jpg "Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 NSP10 Antibody [NBP3-13475]")
Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 NSP10 Antibody [NBP3-13475]
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 NSP10 Antibody [NBP3-13475] - SARS-CoV-2 (COVID-19) nsp10 antibody detects SARS-CoV-2 (COVID-19) nsp10 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp10 stained by SARS-CoV-2 (COVID-19) nsp10 antibody (NBP3-13475) diluted at 1:2000.Applications for SARS-CoV-2 NSP10 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 NSP10
References
1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4
2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9
3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39
4. Khan, M. T., Irfan, M., Ahsan, H., Ahmed, A., Kaushik, A. C., Khan, A. S., Chinnasamy, S., Ali, A., & Wei, D. Q. (2021). Structures of SARS-CoV-2 RNA-Binding Proteins and Therapeutic Targets. Intervirology. https://doi.org/10.1159/000513686
Alternate Names
GFL, Growth factor-like peptide, Non-structural protein 10
Gene Symbol
ORF1ab
Additional SARS-CoV-2 NSP10 Products
Product Documents for SARS-CoV-2 NSP10 Antibody - BSA Free
Product Specific Notices for SARS-CoV-2 NSP10 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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