SARS-CoV-2 NSP10 Alexa Fluor™ Plus 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB11049AFP594

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FAB11049AFP594-100UG
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1049919

View all NSP10 Antibodies »

Product Specifications

Immunogen

E. coli-derived SARS-CoV-2 NSP10 protein

Specificity

Detects SARS-CoV-2 NSP10 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NSP10

Non-structural protein 10 (NSP10), is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP10 is a small 139 amino acid protein that forms independent dodecameric structures composed of four identical trimers where each monomer is capable of binding two zinc ions (2, 3) that are coordinated uniquely and has exposed basic residues on the protein's surface consequently allowing RNA-binding (3). The NSP10 sequence is conserved across coronaviruses (4) and is thought to serve as an important multifunctional cofactor in viral replication. NSP10 was shown to interact with other NSP proteins including NSP14 and NSP16 (5). The N-terminus of NSP10 has been shown to interact with NSP14 and proposed to stabilize and regulate the 3'-5' exoribonuclease domain activity while not directly impacting the methyltransferase activity of NSP14 (6,7). A similar region of NSP10 interacts with NSP16; this interaction is required for stabilization of NSP16 and stimulation of its 2'-O-methyltransferase activity (4,7). Consequently, NSP10 is critically involved in the formation of functional viral mRNA capping machinery in coronaviruses and a potential target to reduce viral replication and pathogenesis (8,9). 

References

  1. Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59. 
  2. Matthes, N. et al. (2006) FEBS Lett. 580:4143.
  3. Su, D. et al. (2006) J. Virol. 80:7902.
  4. Rosas-Lemus, M. et al. (2020) bioRxiv. In press.
  5. Pan, J. et al. (2008) PLoS One 3:e3299.
  6. Bouvet, M. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:9372.
  7. Bouvet, M. et al. (2014) J. Biol. Chem. 289:25783.
  8. Ma, Y. et al. (2015) Proc. Natl. Acad. Sci. 112:9436.
  9. Wang, Y. et al. (2015) J. Virol. 89:8416.

Long Name

Non-Structural Protein 10

UniProt

YP_009725306.1

Additional NSP10 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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